منابع مشابه
Rapid detection of epidermal growth factor receptor mutations in lung cancer by the SMart-Amplification Process.
PURPOSE A positive response to gefitinib in non-small cell lung cancer (NSCLC) has been correlated to mutations in epidermal growth factor receptor (EGFR) gene. Previous reports have been based mainly on diagnostic screening by sequencing. However, sequencing is a time-consuming and complicated procedure, not suitable for routine clinical use. EXPERIMENTAL DESIGN We have developed rapid, simp...
متن کاملUse of a competitive probe in assay design for genotyping of the UGT1A1 *28 microsatellite polymorphism by the smart amplification process.
A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS). However we report here that use of SMAP-2 for polymorphism determination of the UGT1A1 *28 allele required a further ancillary approach for complete background suppression. The UGT1A1 *28 allele is...
متن کاملClinical screening assay for EGFR exon 19 mutations using PNA-clamp smart amplification process version 2 in lung adenocarcinoma.
The presence of EGFR mutations is correlated with a positive therapeutic response to tyrosine kinase inhibitors; therefore, the accurate detection of EGFR mutations is crucial when deciding appropriate therapeutic strategies. Recently, the rapid and sensitive assay smart amplification process version 2 (SmartAmp2) was developed. However, this method can only detect one type of mutation in EGFR ...
متن کاملCharacterization of Schistosome Tegumental Alkaline Phosphatase (SmAP)
Schistosomes are parasitic platyhelminths that currently infect over 200 million people globally. The parasites can live for years in a putatively hostile environment - the blood of vertebrates. We have hypothesized that the unusual schistosome tegument (outer-covering) plays a role in protecting parasites in the blood; by impeding host immunological signaling pathways we suggest that tegumenta...
متن کاملAcoustic Forest for SMAP-Based Speaker Verification
In speaker verification, structural maximum-a-posteriori (SMAP) adaptation for Gaussian mixture model (GMM) has been proven effective, especially when the speech segment is very short. In SMAP adaptation, an acoustic tree of Gaussian components is constructed to represent the hierarchical acoustic space. Until now, however, there has been no clear way to automatically find the optimal tree stru...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 2008
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.52.183